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transfection a498 human renal cancer cell line  (ATCC)


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    ATCC transfection a498 human renal cancer cell line
    INI1 influences CXCL12/CXCR4/CXCR7 chemokine axis. A. INI1-FLAG overexpression in <t>A498</t> cells, which correspond with ccRCC grade 2. As a loading control membrane stained with TCE (stain free) was used. B. Western blot with anti-INI1 antibody demonstrating increase of INI1 abundance in A498 cell line overexpressing INI1 protein. As a loading control membrane stained with TCE (stain free) was used. C. qRT-PCR analysis for CXCR4, CXCR7 and SMARCB1 (endogenous transcript) genes expression in A498 cell lines overexpressing INI1 protein. D. Model describing self-regulation of the SMARCB1 gene by INI1 protein and the interdependence of INI1, AR pathway and CXCL12/CXCR4/CXCR7 axis.
    Transfection A498 Human Renal Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+a498+human+renal+cancer+cell+line/A-498/pmc05714755-114-3-12
    Average 97 stars, based on 1673 article reviews
    transfection a498 human renal cancer cell line - by Bioz Stars, 2026-10
    97/100 stars

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    1) Product Images from "Evaluation of the role of downregulation of SNF5/INI1 core subunit of SWI/SNF complex in clear cell renal cell carcinoma development"

    Article Title: Evaluation of the role of downregulation of SNF5/INI1 core subunit of SWI/SNF complex in clear cell renal cell carcinoma development

    Journal: American Journal of Cancer Research

    doi:

    INI1 influences CXCL12/CXCR4/CXCR7 chemokine axis. A. INI1-FLAG overexpression in A498 cells, which correspond with ccRCC grade 2. As a loading control membrane stained with TCE (stain free) was used. B. Western blot with anti-INI1 antibody demonstrating increase of INI1 abundance in A498 cell line overexpressing INI1 protein. As a loading control membrane stained with TCE (stain free) was used. C. qRT-PCR analysis for CXCR4, CXCR7 and SMARCB1 (endogenous transcript) genes expression in A498 cell lines overexpressing INI1 protein. D. Model describing self-regulation of the SMARCB1 gene by INI1 protein and the interdependence of INI1, AR pathway and CXCL12/CXCR4/CXCR7 axis.
    Figure Legend Snippet: INI1 influences CXCL12/CXCR4/CXCR7 chemokine axis. A. INI1-FLAG overexpression in A498 cells, which correspond with ccRCC grade 2. As a loading control membrane stained with TCE (stain free) was used. B. Western blot with anti-INI1 antibody demonstrating increase of INI1 abundance in A498 cell line overexpressing INI1 protein. As a loading control membrane stained with TCE (stain free) was used. C. qRT-PCR analysis for CXCR4, CXCR7 and SMARCB1 (endogenous transcript) genes expression in A498 cell lines overexpressing INI1 protein. D. Model describing self-regulation of the SMARCB1 gene by INI1 protein and the interdependence of INI1, AR pathway and CXCL12/CXCR4/CXCR7 axis.

    Techniques Used: Over Expression, Control, Membrane, Staining, Western Blot, Quantitative RT-PCR, Expressing

    Related Articles

    Cell Culture:

    Article Title: Evaluation of the role of downregulation of SNF5/INI1 core subunit of SWI/SNF complex in clear cell renal cell carcinoma development
    Article Snippet: .. Cell culture and transfection A498 human renal cancer cell line (obtained from ATCC) was grown in RPMI 1640 (Biowest) with 10% Fetal Bovine Serum (FBS) (Biowest) and 1% penicillin/streptomycin (Gibco) in humidifier incubator (37°C and 5% CO 2 ) according to manufacturer instruction. .. Cloning and overexpression of INI1 protein in A498 cell line cDNA coding for INI1 protein with FLAG-tag obtained from Addgene collection (Plasmid #1953) in vector pFastBac1 INI1 provided by Robert Kingston [ 20 ] was moved on EcoRI restriction site into pcDNA3.1 mammalian expression vector.

    Transfection:

    Article Title: Evaluation of the role of downregulation of SNF5/INI1 core subunit of SWI/SNF complex in clear cell renal cell carcinoma development
    Article Snippet: .. Cell culture and transfection A498 human renal cancer cell line (obtained from ATCC) was grown in RPMI 1640 (Biowest) with 10% Fetal Bovine Serum (FBS) (Biowest) and 1% penicillin/streptomycin (Gibco) in humidifier incubator (37°C and 5% CO 2 ) according to manufacturer instruction. .. Cloning and overexpression of INI1 protein in A498 cell line cDNA coding for INI1 protein with FLAG-tag obtained from Addgene collection (Plasmid #1953) in vector pFastBac1 INI1 provided by Robert Kingston [ 20 ] was moved on EcoRI restriction site into pcDNA3.1 mammalian expression vector.



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    transfection a498 human renal cancer cell line  (ATCC)
    97
    ATCC transfection a498 human renal cancer cell line
    INI1 influences CXCL12/CXCR4/CXCR7 chemokine axis. A. INI1-FLAG overexpression in <t>A498</t> cells, which correspond with ccRCC grade 2. As a loading control membrane stained with TCE (stain free) was used. B. Western blot with anti-INI1 antibody demonstrating increase of INI1 abundance in A498 cell line overexpressing INI1 protein. As a loading control membrane stained with TCE (stain free) was used. C. qRT-PCR analysis for CXCR4, CXCR7 and SMARCB1 (endogenous transcript) genes expression in A498 cell lines overexpressing INI1 protein. D. Model describing self-regulation of the SMARCB1 gene by INI1 protein and the interdependence of INI1, AR pathway and CXCL12/CXCR4/CXCR7 axis.
    Transfection A498 Human Renal Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transfection+a498+human+renal+cancer+cell+line/A-498/pmc05714755-114-3-12
    Average 97 stars, based on 1 article reviews
    transfection a498 human renal cancer cell line - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

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    INI1 influences CXCL12/CXCR4/CXCR7 chemokine axis. A. INI1-FLAG overexpression in A498 cells, which correspond with ccRCC grade 2. As a loading control membrane stained with TCE (stain free) was used. B. Western blot with anti-INI1 antibody demonstrating increase of INI1 abundance in A498 cell line overexpressing INI1 protein. As a loading control membrane stained with TCE (stain free) was used. C. qRT-PCR analysis for CXCR4, CXCR7 and SMARCB1 (endogenous transcript) genes expression in A498 cell lines overexpressing INI1 protein. D. Model describing self-regulation of the SMARCB1 gene by INI1 protein and the interdependence of INI1, AR pathway and CXCL12/CXCR4/CXCR7 axis.

    Journal: American Journal of Cancer Research

    Article Title: Evaluation of the role of downregulation of SNF5/INI1 core subunit of SWI/SNF complex in clear cell renal cell carcinoma development

    doi:

    Figure Lengend Snippet: INI1 influences CXCL12/CXCR4/CXCR7 chemokine axis. A. INI1-FLAG overexpression in A498 cells, which correspond with ccRCC grade 2. As a loading control membrane stained with TCE (stain free) was used. B. Western blot with anti-INI1 antibody demonstrating increase of INI1 abundance in A498 cell line overexpressing INI1 protein. As a loading control membrane stained with TCE (stain free) was used. C. qRT-PCR analysis for CXCR4, CXCR7 and SMARCB1 (endogenous transcript) genes expression in A498 cell lines overexpressing INI1 protein. D. Model describing self-regulation of the SMARCB1 gene by INI1 protein and the interdependence of INI1, AR pathway and CXCL12/CXCR4/CXCR7 axis.

    Article Snippet: Cell culture and transfection A498 human renal cancer cell line (obtained from ATCC) was grown in RPMI 1640 (Biowest) with 10% Fetal Bovine Serum (FBS) (Biowest) and 1% penicillin/streptomycin (Gibco) in humidifier incubator (37°C and 5% CO 2 ) according to manufacturer instruction.

    Techniques: Over Expression, Control, Membrane, Staining, Western Blot, Quantitative RT-PCR, Expressing